Reference Measurement Services

Reference Measurement Services

The United Kingdom Accreditation Services (UKAS) has accredited the Weqas Reference Measurement Laboratory to ISO/IEC 17025:2017 (General requirements for the competence of testing and calibration laboratories) in accordance with the requirements of BS EN ISO 15195:2018 (Laboratory medicine – Requirements for the competence of calibration laboratories using reference measurement procedures).

Weqas Reference Measurement has two main roles:

  • To develop primary and/or secondary reference methods in order to give stated, traceable, analyte values with known uncertainty in calibrator, QC and EQA material used within Laboratory Medicine. This service is available to QC manufacturers, IVD manufacturers and EQA providers.
  • To produce low cost Quality Control matrix reference material (QCRM).

The Reference Measurement Laboratory undertakes network analyses as part of the initiative undertaken by the Joint Committee on Traceability in Laboratory Medicine (JCTLM) and has also been involved in SRM targeting exercises in conjunction with the Institute for Reference Materials and Measurements (IRMM).
All analyses utilise state of the art technology with methods validated according to the guidelines of ISO 15195 (Laboratory Medicine – Requirements for reference measurement laboratories). Traceable reference material and gravimetric preparation of samples is used where possible, allowing the establishment of uncertainty measurements to be estimated according to GUM.



The Goals of a reference system are:

  • To establish worldwide compatibility of clinical measurements
  • To evaluate the performance of routine laboratories on an objective basis within systems of QC and QA
  • To permit the evaluation of accuracy of analytical systems for routine use

Definition of a reference method

  • A method which after exhaustive investigations has been shown to have negligible inaccuracy in comparison with its imprecision.

ButtnerJ et al. Clin Chem Clin Biochem 1980; 18:69-77.

  • A thoroughly investigated method, clearly and exactly describing the necessary conditions and procedures, for the measurement of one or more property values that has been shown to have accuracy and precision commensurate with its intended use and that can therefore be used to assess the accuracy of other methods for the same measurement, particularly in permitting the characterization of a reference material.

International Organization for Standardization. ISO Guide 30. 2nd Ed. Geneva: ISO, 1992.

  • A technique that can be accomplished with generally available equipment and that, after exhaustive investigation, has been shown to have acceptable known inaccuracy in comparison with a definitive method.


The Reference Measurement Laboratory currently offers reference services using a number of analytical techniques approved as reference methods by the JCTLM. These services are listed on JCTLM website.

We also supply Quality Control Reference Material (QCRM). For details of the material available, please see the QCRM section.

Measurands for which reference values are currently offered, UKAS accredited reference services indicated by *.

Measurement TechniqueMeasuandLink to further details
Liquid Chromatography Tandem Mass SpectrometrySteroids
- Oestradiol
- Progesterone
- Testosterone*
- Cortisol*
LC-MS/MS Steroids
Gas Chromatography Mass SpectrometrySteroids
- Oestradiol
- Progesterone
- Testosterone*
- Cortisol*
GCMS Steroids
Gas Chromatography Mass SpectrometryBile Acids
- Cholic Acid
- Deoxycholic Acid
- Chenodeoxycholic Acid
GCMS Bile Acids
Gas Chromatography Mass SpectrometryCreatinine*
Uric Acid*
GCMS Creatinine
GCMS Glucose
GCMS Uric acid
GCMS Cholesterol
GCMS Triglycerides
Flame Atomic Absorption / Emission SpectrometrySodium*
UV / Vis spectrometryLactate Dehydrogenase*
Gamma Glutamyl Transferase*
Aspartate Transaminase*
Alanine Transaminase*
UV / Vis Spec