LC-MS/MS – Steroids

LC-MS/MS – Steroids

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Measurands currently available include: Cortisol and Testosterone. A Progesterone method is currently awaiting validation and Oestradiol will be developed shortly.

Traceability - Serum/Plasma (urine - cortisol)

MeasurandStandard (certfied purity)Control Material
OestradiolNMIS CRM 6004-9SRM 576, 577, 578
ProgesteroneNMIS CRM 6003-9SRM 347, 348
TestosteroneM914 (AGAL) >99%NIST 971
CortisolSRM 921 SRM 192, 193

Measurement Method Details

Measurand / Internal Standard Sample
pre-treatment
Transitions
Steroid
Parent
Steroid
Daughter
Internal
Standard
Parent
Internal
Standard
Daughter
Cortisol
[2,3,4 - 13C3] Cortisol
Liquid-liquid extraction
(methyl tertiary butyl ether)
363.3121.2, 97.15366.3124.2, 100.15
Testosterone
[2,3,4 - 13C3] Testosterone
Liquid-liquid extraction
(ethyl acetate / hexane)
289.097.0, 109.0292.1100.0, 112.0
Progesterone
[2,3 - 13C2] Progesterone
Liquid-liquid extraction
(hexane)
315.197.1, 109.0317.299.0, 111.0