GCMS – Cholesterol

GCMS – Cholesterol

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Metrological traceability is a requirement for ISO 15189 accreditation and laboratories are now becoming aware of the need for traceability of their routine methods. Reference values for total cholesterol are assigned by a CDC laboratory using the Abel Kendell method. The Weqas Reference Laboratory utilises the NIST Isotope Dilution Gas Chromatography Mass Spectrometry (ID-GCMS) Reference Method to assign target values for Weqas Lipid EQA material, providing the “true” measurement of Cholesterol, ensuring the transfer of accuracy from primary reference methods to routine methods. Use of reference targeted material is recommended for standardising results and highlighting any bias due to non-specificity of routine methods.

ID-GCMS  for Cholesterol is assigned to Lipid EQA material, providing a “true” assessment for accuracy of participant returned results to the Reference method for each distribution. This service is also available to external clients in the post-marketing vigilance of the EU Directive 98/79/EC.


MeasurandStandard (certfied purity)Control Material
CholesterolNIST 911c (99.2%)NIST 909c

Measurement Method Details

Performance Criteria
Measurement Range1.0 - 8.0 mmol/L
Maximum allowable %CV1.5 %
Maximum bias (control)0.6 %
Method uncertainty1.8 %

JCTLM listed Reference Measurement Service for Cholesterol: