GCMS – Steroids

< Go back to Reference Laboratory Services page

Analytes currently available include: Progesterone, Cortisol, Testosterone. An Oestradiol method is currently awaiting validation.

The progesterone and cortisol GCMS methods were developed in collaberation with Professor Linda Thienpoint, University of Ghent and optimised for our working practices.

Traceability - Serum/Plasma (urine - cortisol)

Analyte Standard (certfied purity)Control Material
OestradiolSigma >99%SRM 576, 577, 578
ProgesteroneSigma >99%SRM 347
TestosteroneM914 (AGAL) >99%"In house"
CortisolSRM 921 SRM 192, 193

Analytical Method Details

Analyte / Internal Standard Sample pre-treatmentDerivative

17ß Oestradiol-
[2,4,16,-d4] oestradiol

Liquid-liquid extraction (diethyl ether)Heptafluorobutyrate
Progesterone
[2,3,C13] Progesterone
Liquid-liquid extraction (hexane)
Reverse phase HPLC
Heptafluorobutyrate
Testosterone
[16,16,17-d4] Testosterone
Liquid-liquid extraction (hexane)
Gel Chromatography
Heptafluorobutyrate
Cortisol
[1,2-d4] Cortisol
Liquid-liquid extraction (dichloromethane)
Gel Chromatography
Heptafluorobutyrate

Accreditation

UKAS Proficiency Testing 4301UKAS Calibration 4301


Events

  1. AACC Annual Conference – Chicago

    31st July - 2nd August
  2. Harrogate EQA Workshop

    20th September