GCMS Bile Acids
Current immunoassays for Bile acids measure the total bile acids present. Monitoring each of the individual major bile acids will enable the significant biases and differences observed between each of the methods to be addressed 
| Analyte | Standard (certfied purity) | Control Material |
|---|---|---|
| Cholic Acid | Sigma >99% | "In house" |
| Deoxycholic Acid | Sigma >99% | "In house" |
| Chenodeoxycholic Acid | Sigma >99% | "In house" |
| Analyte / Internal Standard | Sample pre-treatment | Derivative |
|---|---|---|
|
Cholic Acid |
Solid phase extraction Gel Chromatography |
Silyl methyl ether |
| Deoxycholic acid [2,2,4,4,-d4]-deoxycholic acid |
Solid phase extraction Gel Chromatography |
Silyl methyl ether |
| Chenodeoxycholic acid [2,2,4,4,-d4]-chenodeoxycholic acid |
Solid phase extraction Gel Chromatography |
Silyl methyl ether |
GCMS Targets of Cholic Acid, Chenodeoxycholic Acid and Deoxycholic Acid for Comparison of Total bile Acids in Serum. 130k